Flaky
A red, nervous porcupine with dandruff on her quills (hence the name "Flaky"). She is usually very nervous due to a fear of dying. She is also shown to be a coward and is highly unstable. For a long time, her gender was never made clear, though hints always leaned toward her being female.

The Mole

A blind lavender mole with black glasses, a black trench coat, a cane, and a mole near his nose. Due to his blindness, some of his actions get him and the other Tree Friends killed. He often does things blind people shouldn't be doing, so as reading and driving. His arch enemy is The Rat.

Disco Bear

A golden bear with an afro and '70s style clothing. He is also fat as pointed out in Ipso Fatso. In most episodes he is seen trying to flirt with girls such as Petunia and Giggles, but they simply find him a nuisance

Russell

A turquoise sea otter with pirate accessories. He has the overall apperance of a pirate but after Season 1 he lost his five o-clock shadow. He is often seens doing water-related activities.

Lifty & Shifty

Lifty & Shifty are green kleptomaniac raccoons who steal whatever they can when possible. You can tell them apart by Lifty being naked and Shifty wearing a green fedora. Shifty was the first Tree friend to be created, way before Cuddles. Even though they are brothers, they get along poorly sometimes, which often results in the failure of their plans (especially by them dying). They have the lowest survival rate of all the characters

Mime

A purple reindeer with a mime-like painted face and striped shirt. He is meant to be entertaining but results in others around him getting killed or getting himself killed. He normally rides a unicycle while juggling three balls. He does not talk because he is a mime, which normally results to the tree friend he's communicating to not to understand him, which in some cases cause many deaths, for example the episode Who's to Flame? Where he is trying to tell the fire department that the house is burning down but since the're talking over the phone fireman Lumpy can't understand him and hangs up (which later the mime sees firemen and tries to communicate with them yet they still don't understand).

Cro-Marmot

A green marmot frozen in a block of ice who wears leopard-skin loincloth and holds a club. He is frozen in an iceblock and lives in an apartment that is mostly ice. Despite being frozen, he can perform many tasks when offscreen. He often drives around in an ice cream truck.

Flippy

A green bear with army clothing. Whenever sounds are heard by him that remind him of war, he flips out which results in everyone's death. He is the most famous character in the series.

Splendid

A blue flying squirrel with a red superhero mask. He causes more harm than good and has similarities to Superman, like being allergic to kryptonut.

Lammy

Lammy is a purple sheep with a purple bow and a white jumper representing her wool. She is shown to be schizophrenic due to Mr. Pickels' actions resulting in her getting everyone killed.

Mr. Pickels

Mr. Pickels is a pickle with a tophat and moustache. He is similar to Flippy's dark side due to a likeliness of killing everyone he is near. He is also Lammy's 'imaginary' friend that only she can see in animated form. However, in Royal Flush, Giggles sees him. Mr. Pickels is a small imaginary friend of Lammy. He has a moustache and a top hat. Lammy, at first, thinks of him as nice, but Mr. Pickels then starts causing chaos, from ripping the head of a teddy bear off to running over Handy with his own truck.